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At GenScript, our Oligo Pool service offers researchers a comprehensive solution for customized oligonucleotide pools. Using our proprietary high density Complementary Metal Oxide Semiconductor (CMOS) technology, we achieve unmatched precision via microelectrode control, ensuring even distribution and minimal batch differences. Harnessing the industry's largest capacity, with over 8 million oligos on a single chip, our technology empowers breakthroughs in gene editing, protein/antibody engineering, DNA data storage, and more.
Consistency
Complete chamber reaction for excellent uniformity
Precision
Precise control of current and voltage at each electrode
Capacity
Up to 8 Million Oligos on a single chip
Discover how our Next-Generation CMOS Technology is pushing the boundaries of DNA Synthesis.
Miniature Semiconductor Chip (MSC) enables large-scale de novo synthesis of DNA using proprietary electrochemistry technologies and delivers precision on a microelectrode scale.
Less interference of H2O, O2, O3 and static electricity
Full chamber reaction with standard wafer and chip production process
| High Fidelity (HIFI 92K) | High Density (HD 8M) | |
|---|---|---|
| Oligo length | 20-170nt | 20-170nt |
| Chip Size | Up to 92K on 1 Chip | Up to 8M on 1 Chip |
| Yield | Up to 1 fmol for each oligo | |
| Turnaround Time | 3-5 business days* | |
| Quality control |
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*PCR amplification of entire pool, cloning, and sequencing for sequence coverage and accuracy at an additional fee
*GenScript Internal Data 2025. Time frame of 3-5 business days for GenScript Oligo Pools is based on internal production data available as of 2025.
Genome Editing
CRISPR gRNA Library
Oligo pool gRNA libraries, utilizing CRISPR gene editing, enable high-throughput screening for cancer therapy, diagnostics, and microorganism engineering. They facilitate gene knock-out and activation, probing tumorigenesis, biomarkers, and metabolic pathways. This transformative tool leverages CRISPR's precision for versatile biomedical and biotech applications.
NGS Target Enrichment
NGS Target Enrichment
Next-generation sequencing (NGS) target enrichment utilizing oligo pools has emerged as a transformative technique in genomics research. By custom designing pools of oligonucleotide probes complementary to specific genomic regions of interest, researchers can efficiently capture and amplify relevant DNA fragments for sequencing. This approach not only streamlines the sequencing process but also significantly reduces the cost and data burden.
Protein & Antibody Engineering
Mutant Libraries
Mutant libraries are powerful, high-throughput tools for optimizing protein structure and function. However, traditional methods for library construction, including error-prone PCR or degenerate methods, suffer from limited control over codon usage and poor variant representation. Powered by our semiconductor-based oligo synthesis technology, our Precision Mutant Library service offers precise control over each synthesized variant. The result is a more diverse and fully covered mutant library with unbiased distribution.
Gene Fragments
Gene Fragments
Gene Fragments offer a cost-effective, swift, and effective avenue for constructing the requisite genes in your research endeavors. These double-stranded DNA fragments of up to 3,000 base pairs in length, fulfilling the demands of easy gene construction or modification suitable for a variety of applications. The Gene Fragments Service leverages our exclusive complementary metal oxide semiconductor (CMOS) integrated circuits (IC), ensuring unmatched precision, accelerated delivery schedules, and exceptional cost-effectiveness.
DNA Data Storage
DNA Data Storage
DNA data storage involves encoding digital information as DNA sequences, utilizing the four DNA bases (A, T, C, and G) to represent binary data (0s and 1s). These sequences can be later synthesized into DNA strands for storage. Oligo pools are used to generate these sequences efficiently and in parallel, allowing large amounts of data to be encoded using unique combinations of oligos.
sgRNA Library Application Note
Building a diverse CRISPR gRNA library using arrayed-synthesized oligo pools.
NGS Target Enrichment Application
Double stranded DNA capture probes in NGS targeted sequencing.
Protein & Antibody Engineering Application
Assembly of precise mutant libraries using arrayed-synthesized oligos.
Oligo Pools Brochure
Learn how GenSript Oligo Pool enables precise, scalable library construction and high-throughput screening.
Pricing depends on the complexity tier (Pool Size) and the oligo length tier (20-79nt, 80-109nt,110-130nt, 131-150nt) of the oligo pools.
If you need to contact your Account Manager regarding pricing, their contact information is available under your account or email us at [email protected]
GenScript can synthesize Oligos from 20 nucleotides up to maximum of 170 nucleotides.
GenScript Oligo Pool offers a default QC option using PCR amplification of spike-in control oligos synthesized on the same chip. Each pool includes 50 optimized control oligos that are amplified to confirm full-length synthesis accuracy. Customer sequences are screened to ensure QC primers only target these control oligos.
Synthesis verification of full oligo length using spike-in control oligos New
In order to verify full-length synthesis of oligos, we now offer an additional QC option for FREE, PCR amplification of control oligos synthesized on the same chip . Sequences of 50 optimized control oligos will be synthesized with each pool and PCR amplification of the control oligos will be performed to confirm oligo synthesis. Customer sequences will be screened to ensure QC PCR primers will only capture control oligos.
Synthesis Verification of Full Length Oligos in a Pool
Yes. GenScript Oligo Pools platform are allowed to synthesis one additional type of degenerate base (R, Y, S, W, K, M, B, D, H, V, N) according IUPAC (International Union of Pure and Applied Chemistry).
Yes. You can download the GenScript’s Oligo Pool User Guide and PCR Amplification Guidelines to learn about PCR enrichment and amplification best practices for Oligo Pools.
Modification is non-standardized offering with Genscript Oligo Pools.
If you need customization, please contact your Account Manager regarding pricing, their contact information is under your Account.
Oligo pools are collections of oligonucleotides synthesized using GenScript’s proprietary high density Complementary Metal Oxide Semiconductor (CMOS) technology. GenScript oligo pools are designed for PCR (Polymerase Chain Reaction) amplification, which requires that the oligos be carefully designed with specific sequences at their ends to ensure effective primer annealing and successful amplification.
GenScript guarantees that when an oligo pool is appropriately designed and used as a template for PCR with suitable primers, it will produce a PCR product of the correct size. However, it's important to note that while general PCR guidelines are provided, modifications to the PCR protocols might be necessary to accommodate specific variables, such as the choice of primers and the presence of certain sequence motifs. As every pool is uniquely made according to the customer’s design, GenScript cannot commit to specific characteristics that could be influenced by the sequence design or downstream workflow.
