Monoclonal Antibody Development Service

Key Service Features

Services

GenScript has a proven track record of successful custom mAb generation and utilizes a range of proprietary technologies to maximize your chances of generating mAbs of high specificity and affinity. Our custom mAb services can be adapted to cater to all levels of project complexity.

How to Choose Suitable Monoclonal Antibody Service

Our customizable service options can handle all levels of project complexity. Whether you have a simple or challenging antigen, need a few or many mAb clones, or have specific screening requirements, rest assured that our antibody experts will do their best to tailor a custom mAb project to perfectly suit your research requirements.

Get started with our service selection guide below, or get in touch with us here.

Project complexity and Recommended mAb generation service

YOU TELL US

Antigen information

Desired mAb clone number

Target research application

WHAT WE WILL DO

Antigen design & production (optional)

Develop project-specific mAb generation strategy

Application-tailored mAb clone screening

Case Studies

• Custom mouse monoclonal antibodies

  Case Study: Academic customer was seeking to develop an agonistic antibody against a difficult antigen – a single transmembrane cell surface protein.

  
• MonoRab™ custom rabbit monoclonal antibodies

  Case Study 1: MonoRab™ was used to develop a high affinity, low cross-reactivity antibody against the small molecule Ractopamine.

  

  

  

  The anti-Ractopamine rabbit MonoRab™ mAb showed very low affinity against very similar drugs (A) Salbutamol (Sal; IC50=173.7; cross reactivity rate=0.0304%), and (B) Clenbuterol (Cle; IC50=160.7; cross reactivity rate=0.0329%), but (C) very high affinity (IC50=0.05) against Rac.

  Case Study 2: MonoRab™ was used to develop an anti-FLAG tag antibody for Western blot and IP applications.

  

  The rabbit anti-FLAG mAb generated using MonoRab™ demonstrated higher specificity and affinity than the corresponding anti-FLAG mouse mAb.

  Lane A: MonoRab™ mAb; C-terminal flag tag fusion protein
  Lane B: Mouse mAb; C-terminal flag tag fusion protein
  Lane C: MonoRab™ mAb; N-terminal flag tag fusion protein
  Lane D: Mouse mAb; N-terminal flag tag fusion protein
  Lane E: MonoRab™ mAb; Mid-terminal flag tag fusion protein
  Lane F: Mouse mAb; Mid-terminal flag tag fusion protein
  

  Case Study 3: MonoRab™ was used to develop antibodies for IHC

  

  Comparison of Rabbit and Mouse anti-β Actin mAb's in monkey lung.

  GenScript generated mouse anti-beta actin mAb was visualized via anti-mouse 568 (A) and directly compared with GenScript generated rabbit anti-β actin visualized with anti-rabbit FITC (B). The resulting co-localization image (C) revealed significantly more binding of rabbit anti-beta actin than mouse, as highlighted by the white arrows, indicating that the rabbit anti-β actin mAb has a much higher affinity than its mouse generated counterpart.

  

  Comparison of GenScript Rabbit Anti-Beta Actin mAb with Competitors in monkey cardiac muscle.

  GenScript generated rabbit anti-beta actin mAb (A) was compared with competitor generated rabbit anti-beta actin mAb (B&C) via Nova-red visualization. The resulting images reveled that compared with competitor B, GenScript rabbit mAb showed significantly higher sensitivity as shown by the blue arrows. Compared with competitor C, Genscript's rabbit mAb showed higher specificity, as competitor C's mAb bound to other forms of actin present within the cardiac tissue, as shown via the yellow arrows.

Review some of our helpful antibody-related technical resources here!

Additional Services

We also offer specialized services such as phospho-specific and anti-idiotype antibody development.

Quotation and Ordering

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.