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![GenCRISPR gRNA & HDR Template Design Tools]( "GenCRISPR gRNA & HDR Template Design Tools")
The GenCRISPR gRNA Design Tools makes guide RNA and HDR template design easy – Simply select your experiment type to get started.
Start Your ProjectNote: Selecting HDR knock-in will enable HDR template design options
Why choose our CRISPR design tools?
sgRNA design tool
- Comprehensive species options, updated on-target and off-target scores
- Designs target early exons to avoid truncated functional proteins
- Higher transcript coverage
- Ideal GC% for sgRNA
- Validated KO% up to 97%, more detail>>
- Design Tool User Guide
HDR knock-in template design tool
- Supports 1-1500bp deletions, 1bp-20kb insertions
- Linear ssDNA / linear closed-end dsDNA / circular dsDNA formats available
- CTS option and updated on-target and off-target scores increase editing efficiency
- Multiple sequence check function helps avoid mistakes
- Design Tool User Guide
pegRNA design tool
- Comprehensive Functions: Simply enter your desired gene symbol or sequence for guided support from design to ordering.
- More Editing Approaches: Supports knock-ins, knockouts, and sequence replacement, with knock-ins up to 100 nt.
- Enhances Editing Efficiency: Includes nicking sgRNA, 3' extension, and silent mutations.
- Easy to Use: Displays base and amino acid changes, with multiple indices for easy selection.
- Design Tool User Guide
How do GenScript’s CRISPR design tools compare to other available design tools?
GenScript CRISPR Design Tools Synthego CRISPR Design Tool IDT Alt-R™ CRISPR Design Tools Editing Systems Supported Cas9, Cas12a, prime editing Cas9 only Cas9 only Multiple mammalian and bacterial species supported Yes Yes Yes On-/Off-target Scoring Advanced Basic Advanced Edit Types Supported Knockout, knock-in, sequence replacement Knockout only Knockout, knock-in HDR Template Design Options Three modalities None Two modalities Plasmid Design Options Yes No No Designs for Optimized GC Content Yes No No Targets Early Exons Yes No No Optimized Transcript Coverage Yes No No Purification Options Desalt and HPLC Desalt only Desalt only 
Easily design compatible
HDR templates & sgRNAsAutomatically optimize
homology arm design
Select from ssDNA
or dsDNA formatsCreate an SNP or insert
any sequence up to 20 kb
Streamline your HDR
template & sgRNA orderssgRNA/template pairs are
ranked by algorithm scoresInteractive Design Map
How to Design CRISPR Knock-in sgRNA + HDR Templates
Start Your ProjectNote: Selecting HDR knock-in will enable HDR template design options
Other CRISPR gRNA Design Tool And Database
Broad gRNA Design Tool for Any Sequence
- sgRNA designed for any DNA sequence you input
- Algorithm developed and validated by Feng Zhang lab, Broad Institute
Broad sgRNA Database
- Genome-wide gRNA databases for CRISPR editing and transcription activation
- Pre-validated gRNA sequences design by the Broad Institute Ready for delivery; Full-Genome Coverage
GenCRISPR Services
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