Targeted LNP Formulation

Targeted LNP Service

Novel LNP formulation

• CX108

• CX175

• SM-CX712

CX108 LNP exhibited comparable in vivo performance to the benchmark SM102 LNP, and demonstrated enhanced expression in primary T cells after CD3 antibody conjugation, highlighting its potential for in vivo CAR-T applications.

Inquire about novel LNP CX108

Novel LNPs

CX175 LNP exhibited comparable in vivo performance to the benchmark SM102 LNP, and demonstrated enhanced expression in primary T cells after CD3 antibody conjugation, highlighting its potential for in vivo CAR-T applications.

Inquire about novel LNP CX175

Novel LNPs

SM-CX712 LNP can enhance RNA’s distribution to muscle, and reduce its expression in liver by IM injection

Inquire about novel LNP SM-CX712

Novel LNPs

Quality Control and Specification

Case Studies

• T Cell Targeted LNP: CD3-Ab/LNP
  

  Fluc mRNA(N1-methyl-pseU) was encapsulated with SM102-LNP or SM102-LNP decorated with anti-Human CD3 Antibody  (Clone: OKT3, mAb, Mouse, GenScript, A02199), 100ng of mRNA encapsulated in LNP were incubated with jurkat cells in RPMI140 +10%FBS at 37C for 24 hrs. The expression of Fluc was detected using Luciferase Assay (Promega, E4030).

  

  eGFP mRNA(N1-methyl-pseU) was encapsulated with SM102-LNP or SM102-LNP decorated with anti-Human CD3 Antibody  (Clone OKT3, mAb, Mouse, GenScript), 10-150ng of mRNA encapsulated in LNP were incubated with primary T cells  for 24 hrs. The expression of eGFP was detected using flow cytometry. W1, W2 and W3 indicate different wash conditions.

  

  

  Fluc mRNA(N1-methyl-pseU) was encapsulated with SM102-LNP or SM102-LNP decorated with anti-mouse CD3 Antibody, 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice, after 24 hrs, mice were sacrificed, and the expression of Fluc mRNA was measured with whole body imaging with different organs.

• T Cell Targeted LNP: CD5-Ab/LNP
  

  Resting primary T cells were seeded at 1×10⁶ cells per well in a 24-well plate and transfected with 500 ng of generic LNP, CD3Ab-LNP, or CD5Ab-LNP. T-cell expression was assessed by FACS at 48 h post-transfection. Because CD3Ab (OKT3) can activate primary T cells, CD3Ab-LNP showed a higher expression level compared with CD5Ab-LNP. Notably, despite the difficulty of transfecting resting T cells, CD5Ab-LNP still achieved over 80% transfection-positive cells.

  
• T Cell Targeted LNP: CD4-Ab/LNP & CD8-Ab/LNP
  

  

  Resting primary T cells were seeded at 1×10⁶ cells per well in a 24-well plate and transfected with 500 ng of generic LNP, CD3Ab-LNP, CD4Ab-LNP, or CD8Ab-LNP. T-cell expression was assessed by FACS at 48 h post-transfection. CD4Ab-LNP and CD8Ab-LNP showed selective delivery to CD4⁺ and CD8⁺ T cells, respectively, with minimal off-target expression.

• CD34+ Cell Targeted LNP: CD34-Ab/LNP
  

  EGFP mRNA(N1-methyl-pseU) was encapsulated with SM102-LNP or SM102-LNP decorated with anti-Human CD34 Antibody (Purified anti-human CD34 antibody(Anti-Human CD34 Antibody, mAb, Mouse, GenScript, CP0001) 100ng of mRNA encapsulated in LNP were incubated with Kasumi-1 cells  for 24 hrs. The expression of eGFP was detected using flow cytometry.

  

  EGFP mRNA(N1-methyl-pseU) was encapsulated with ALC0315-LNP or ALC0315-LNP decorated with anti-Human CD34 Antibody (Purified anti-human CD34 antibody(Anti-Human CD34 Antibody, mAb, Mouse, GenScript, CP0001) 100ng of mRNA encapsulated in LNP were incubated with primary human CD34+ cells  for 24 hrs. The expression of eGFP was detected using flow cytometry.

• Liver Targeted LNP: Tri-GalNac/LNP
  

  

  Fluc mRNA(N1-methyl-pseU) was encapsulated with LNP , 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice, after 24 hrs, mice were sacrificed, and the expression of Fluc mRNA was measured with whole body imaging with different organs.

  

  EGFP mRNA(N1-methyl-pseU) was encapsulated with SM102-LNP or SM102-LNP decorated with TriGalNac, 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice, after 24 hrs, mice were sacrificed, and liver organs were lysed. The eGFP expression in lysate was detected using western blot method.

• Liver Targeted LNP: 20DODAP LNP
  

  

  Fluc mRNA(N1-methyl-pseU) was encapsulated with LNP , 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice, after 24 hrs, mice were sacrificed, and the expression of Fluc mRNA was measured with whole body imaging with different organs.

• Spleen Targeted LNP: SM10218PA LNP
  

  

  Fluc mRNA(N1-methyl-pseU) was encapsulated with LNP , 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice, after 24 hrs, mice were sacrificed, and the expression of Fluc mRNA was measured with whole body imaging with different organs.

• Lung Targeted LNP: 50DOTAP LNP
  

  

  Fluc mRNA(N1-methyl-pseU) was encapsulated with LNP , 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice, after 24 hrs, mice were sacrificed, and the expression of Fluc mRNA was measured with whole body imaging with different organs.

• Lung Targeted LNP: CD31-Ab/LNP
  

  

  Fluc mRNA (m1Ψ) was encapsulated with LNP, 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice. Conjugation of anti-CD31 antibodies to LNPs can enhance mRNA expression levels in the lungs of mice. FACS analysis of lung cells revealed that CD31-Ab/LNPs exhibit clear targeting specificity toward CD31⁺ cells.

• Macrophage Targeted LNP: Mannose/LNP
  

  Fluc mRNA (m1Ψ) was encapsulated with LNP, 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice. Mannose/LNP improved Fluc mRNA expression in spleen and lymph node system.

  

  Mannose/LNPs delivering sgRNA targeting Zeb2 achieved efficient gene editing in Cas9⁺ BMDM cells and significantly suppressed tumors in a Cas9-GFP mouse model.

• Kidney enhanced delivery: KKEEE/LNP
  

  

  Fluc mRNA (m1Ψ) was encapsulated with LNP, 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice. KKEEE/SM102 LNP enhanced Fluc mRNA expression in kidney organ ~4.6 times comparing undecorated LNP.

FAQs

• How much payload should I provide for my LNP project?

  The amount of payload required depends on your desired final quantity and whether decoration is needed:

  Final Amount Needed	Without Decoration	With Decoration
  <0.5 mg	3x final amount	4x final amount
  >0.5 mg	2x final amount	4x final amount

  Example: If you need 0.3 mg final LNP without decoration, please provide 0.9 mg of payload (3x). If you need 1 mg final LNP with decoration, please provide 4 mg of payload (4x).

  For customer-provided antibodies: We require 75 nmol of antibody per 1 mg of final RNA/LNP product.

• What types of antibodies can be used for LNP conjugation?

  We accept various antibody formats for targeted LNP conjugation, including:

  • Full-length antibodies (IgG)
  • scFv (single-chain variable fragments)
  • Fab fragments
  • VHH (nanobodies)
  • Other antibody formats

  If you have questions about a specific antibody format, please contact our technical team to discuss compatibility.