Cas Nuclease Product List

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Overview

The simplicity and versatility of CRISPR gene editing technology contribute to its utilization in various downstream applications, including gene and cell therapy, cell line engineering, animal model generation, and in vitro diagnostics. Through our partnership with Feng Zhang's laboratory at the Broad Institute of MIT and Harvard*, GenScript offers validated RUO and GMP grade enzymes for CRISPR gene editing. Extensive selection of Cas9, Cas12a and Cas13a, Prime and Base editing enzyme products. In addition we also offer the mRNA and LNP versions of the enzymes in linear, circular or self-amplifying format. Our complete offering also includes gRNA synthesis and HDR donor template synthesis services, optimization kits and antibodies and easy to use resources to help with leveraging CRISPR gene editing for basic research and clinical manufacturing.

What enzyme is right for your application?

Cas9

Double-stranded
blunt break

Base Editor Adenine

Changes a single base e.g., A→G

Cas12a

Double-stranded
staggered break

Base Editor Cytosine

Changes a single base e.g., C→T

Cas13a

Single-stranded
break

Prime Editor

DNA Editing

CRISPR Gene Editing

Product List

All

Cas9 Nucleases

Cas12a Nucleases

Cas13a Nucleases

Prime Editors

Base Editors

Gene Editing Kits

Cas Nuclease Antibodies

Linear mRNA

LNP

Cas9 Nucleases

Recombinant wild-type S. pyogenes Cas9 nucleases, purified from E. coli expression and contains various nuclear localization sequences (NLSs).

Cat. No.	Product Name	Price
RC00001	GenCRISPR™ NLS-wtSpCas9, his-tag	$150.00
RC00002	GMP GenCRISPR™ NLS-wtSpCas9, his-tag	- 3 mg
Z03386	GenCrispr Cas9 Nuclease	$35.00
Z03390	GenCrispr NLS-Cas9-D10A Nickase	$39.00
Z03393	GenCrispr NLS-Cas9-EGFP Nuclease	$188.00
Z03467	GenCrispr NLS-Cas9-EGFP Nuclease	$241.00
Z03621	GenCRISPR™ Ultra NLS-Cas9-Research	$695.00
Z03622	GenCRISPR™ Ultra eSpCas9-2NLS-Research	$765.00
Z03623-GMP	GenCRISPR™ Ultra NLS-Cas9-GMP	$6900.00
Z03624-GMP	GenCRISPR™ Ultra eSpCas9-2NLS-GMP	$6900.00
Z03699	GenCRISPR™ SaCas9 2NLS Nuclease	$188.00
Z03702	GenCRISPR™ Cas9 v1.2	$188.00

Cas12a Nuclease

Recombinant Acidaminococcus sp. (strain BV3L6) Cas12a (cpf1) nuclease or Lachnospiraceae bacterium ND2006 Cas12a nuclease, purified from E. coli expression, and contains nuclear localization sequences (NLSs).

Cat. No.	Product Name	Price
Z03502	GenCRISPR™ Cas12a (Cpf1) Nuclease	$113.00
Z03753	GenCRISPR™ LbCas12a Nuclease	$189.00
Z03762	GenCRISPR™ ErCas12a Nuclease	$189.00

Cas13a Nuclease

Recombinant Leptotrichia wadei Cas13a nuclease or Leptotrichia buccalis Cas13a nuclease, purified from E. coli expression.

Cat. No.	Product Name	Quantity	Price	Order
Z03486	GenCRISPR™ Cas13a (C2c2) Nuclease		$189.00
Z03742	GenCRISPR™ LbuCas13a Nuclease		$189.00

Prime editor

Cat. No.	Product Name	Price
RC00003	PEmax RNase deletion	$150.00
RC00004	PE2	$400.00 100 μg(10mg/ml)
RC00005	PEmax	$200.00
RC00006	PE6b	$200.00
RC00007	PE6c	$150.00
RC00008	PE6d	$150.00
RC00009	PE7	$220.00

Base Editor

Cat. No.	Product Name	Quantity	Price	Order
RC00010	ABE8e		$200.00

Gene Editing Kits

Cat. No.	Product Name	Quantity	Price	Order
L00689	GenCrispr sgRNA Screening Kit		$166.00 30 reactions

Cas Nuclease Antibodies

Cat. No.	Product Name	Price
A01885	GenCrispr Cas9 Antibody, pAb, Rabbit	$99.00
A01935	GenCRISPR™ SpCas9 Antibody (4A1), mAb, Mouse	$99.00 40 μg
A01936	GenCRISPR™ SpCas9 Antibody (14B6), mAb, Mouse	$99.00 40 μg
A01951	GenCRISPR™ SaCas9 Antibody (11C12), mAb, Mouse	$99.00 40 μg
A01952	GenCRISPR™ SaCas9 Antibody (26H10), mAb, Mouse	$99.00 40 μg

Linear mRNA

Cat. No.	Product Name	Price
RP-A00002	CBEmax mRNA (Cap1, m1Ψ)	$350.00
RP-A00018	eSpCas9 mRNA(Cap1, m1Ψ)	$350.00
RP-A00047	saCas9 mRNA (Cap1, m1Ψ)	$350.00
RP-A00048	SB100 (Cap1, m1Ψ)	$350.00 0.2 mg
RP-A00050	eSpCas9 mRNA(Cap 1,5-MOU)	$350.00
RP-A00053	ABE8e mRNA (Cap1, m1Ψ)	$350.00 0.2 mg
RP-A00055	MAD7 mRNA (Cap1, m1Ψ)	$350.00 0.2 mg
RP-A00060	LbCas12a mRNA V2 (Cap1, m1Ψ)	$350.00
RP-A00062	PE2/PE3 mRNA V2 (Cap1, m1Ψ)	$350.00
RP-A00063	PEmax mRNA V2 (Cap1, m1Ψ)	$350.00
RP-A00064	PiggyBac mRNA V2 (Cap1, m1Ψ)	$350.00 0.2 mg

LNP

Cat. No.	Product Name	Quantity	Price	Order
RP-A00019	GenLNP-A01-eSpCas9 mRNA(m1Ψ)-TRAC sgRNA		$600.00 0.05 mg
RP-A00020	GenLNP-S01-eSpCas9 mRNA(m1Ψ)-TRAC sgRNA		$600.00 0.05 mg

Case Study

GenCRISPR SpCas9 - high editing efficiency

GenCRISPR SpCas9 demonstrated high gene editing efficiency in both in vitro cleavage tests and HEK293T cells.

In vitro cleavage test

Gene knockout efficiency_HEK293 T cell
GenCRISPR SpCas9 - batch-to-batch consistency

Gene knockout efficiency_293 T cells & Jurkat cells

GenCRISPR SpCas9 is used for gene knockout in 293T cells and Jurkat cells. As shown in the figure, the gene knockout efficiency of three batches shows no significant difference.
GenCRISPR SpCas9 - batch-to-batch consistency

GenCRISPR SpCas9 is used for gene knockout in 293T cells and Jurkat cells. As shown in the figure, the gene knockout efficiency is equivalent to that of the leading supplier I.
GenCRISPR eSpCas9 minimum off target effect

The fidelity and editing efficiency of GenCRISPR eSpCas9 Protein were evaluated against wildtype Cas9 on Jurkat cells and primary T cells. GenCRISPR eSpCas9 exhibited a superior ability to reduce off-target edits.

Low off-target effects_Jurkat cells

Low off-target effects_ Primary T cells
GenCRISPR eSpCas9 Shows high editing efficiency comparable to wild-type SpCas9

GenCRISPR eSpCas9 exhibits comparable knockout and knock-in efficiency to the wildtype SpCas9 in both Jurkat cells and primary T cells.

High Knockout_ Jurkat cells and Primary T cell

High Knock-in_ Primary T cell
GenCRIPSR™ Ultra eSpCas9 (mt) batch-to-batch consistency

The GenCRIPSR™ Ultra eSpCas9 (mt) shows high batch-to-batch consistency, with no significant differences in cell viability and consistent knockout/knock-in efficiency across the three batches at any concentration.

KO at TRAC sites in HEK293T cells

GFP KI at RAB11A in Jurkat cells
GenCRISPR eSpCas9 shows lower off-target effects compared to HiFi Cas9

GenCRISPR eSpCas9 exhibits lower off-target effects compared to HiFi Cas9 products from leading suppliers, while its knockout and knock-in efficiencies are equivalent to or exceed those of the leading suppliers.

Low off-target effects_HEK293 T cell

High Knockout efficiency_HEK293 T cell

High Knock-in efficiency_Primary T cell
Base editor (ABE8e)

Gene editing efficiency in Jurkat cells using ABE8e RNP

High A to G conversion across different doses

ABE8e protein effectively knocks out B2M protein
Prime editor

Gene editing efficiency in HEK293T cells using PE7 RNP at 50 pmol

PE7 mediated targeted 5 bp deletions at the HEK3 locus in HEK293 cells

No significant impact on cell viability