PD-L1/B7-H1 hFc Chimera, Human

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

Price	$300.00
Cat. No.	Z05742-100
Size	100 μg 500 μg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

PD-L1/B7-H1 (Phe19-Arg238)_x000D_ Accession # Q9NZQ7-1	hFc
N-term	C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1 EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

52 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Immobilized PD-L1/B7-H1 hFc Chimera, Human, mFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.12 μg/ml determined by ELISA. »

The purity of PD-L1/B7-H1 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »

PD-L1/B7-H1 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

PD-L1/B7-H1 hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-1, His Tag with an affinity constant of 2.20 μM as determined in SPR assay (Biacore T200). »

Background

Target Background	B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
Synonyms	CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.