PEmax mRNA V2 (Cap1, m1Ψ)

Price	$350.00
Cat. No.	RP-A00063-0.2
Size	0.2 mg 1 mg Buy in bulk
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Properties Quality Control Specifications Applications Bottom Notes

Description

The PEmax mRNA expresses an optimized prime editor enzymatic protein, which further increased editing efficiency in synergy with PE4, PE5 and engineered pegRNAs (Chen P, et al. 2021, Cell 184, 5635-5652). After efficient delivery into cells or animals, PEmax mRNA can translate into protein including a nickase and reverse transcriptase function for gene editing in cells.

This mRNA features:
- A Cap 1 structure with high capping efficiency
- Codon optimized using GenScript X5 algorithm to enhance expression efficiency
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response
- GenScript’s patented UTR to enhance translation
- A 100A poly(A) tail to mimic natural mature mRNA

Properties

Form	Liquid
Concentration	1mg/mL
Full mRNA length	6661 nt
Full mRNA Molecular Weight	2.16×10^6 Da
Storage buffer	1mM Sodium citrate, pH 6.5
Storage condition	Store at -20°C for short term (<3 months), store at -80°C for long term.

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Quality Control Specifications

Appearance	Clear and free of foreign particles
RNA Length	Expected size band detected
RNA Content	Target ± 5%
Integrity	≥ 75%
OD260/OD280	1.70 ~ 2.30
Capping Efficiency	≥ 90%
Endotoxin	< 10 EU/mg
pH	Target ± 0.5

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Applications

Transfect PEmax mRNA along with pegRNA and nicking sgRNA (optional) into cells through electroporation, LNP encapsulation or transfection reagent. After 3 days of incubation, extract the genomic DNA and perform PCR. Analyze the resulting PCR products by Sanger sequencing.

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T to A editing using PEmax system in HEK293T cells and primary T cells. X5 represents GenScript codon optimization algorithm. »

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.