dsRNA Standard (Uncapped, unmodified)

dsRNA is prepared by annealing two reverse complementary RNA strands prepared by in vitro transcription. This dsRNA is uncapped. It contains all natural unmodified uridine in the RNA sequence. The dsRNA provides a good control for developing dsRNA assay method, or as controls for testing related antibodies.

Price	$350.00
Cat. No.	RP-A00059-0.2
Size	0.2 mg Buy in bulk
Quantity
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Properties Quality Control Specifications Applications Bottom Notes

Description

Properties

Form	Liquid
Concentration	1mg/mL
Full mRNA length	445 bp
Full mRNA Molecular Weight	286542 Da
Storage buffer	TES buffer: 10mM Tris HCl, pH7.5, 100mM NaCl, 1mM EDTA
Storage condition	Store at -20°C for short term (<3 months), store at -80°C for long term.

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Quality Control Specifications

Appearance	Clear and free of foreign particles
RNA Length	Expected size band detected
RNA Content	Target ± 5%
OD260/OD280	1.70 ~ 2.30
Endotoxin	< 10 EU/mg
pH	Target ± 0.5

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Applications

Serially dilute the dsRNA to the desired final concentration range. Prepare samples and dilute to the desired concentration. Load both dsRNA standard dilutions and samples into a 96-well ELISA plate and perform the assay according to the manufacturer’s instructions.

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Two samples with known dsRNA concentrations were assayed using the dsRNA Standard and a dsRNA ELISA kit. A five-parameter logistic curve was used to generate the standard curve (top). Measured dsRNA concentration was in good agreement with the expected concentrations (respectively 100.8% and 96.4%) (bottom). »

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.