tdTomato mRNA (Cap1, m1Ψ)

Price	$350.00
Cat. No.	RP-A00056-0.2
Size	0.2 mg Buy in bulk
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Properties Quality Control Specifications Applications Bottom Notes

Description

tdTomato mRNA encodes for a red fluorescent protein derived from corals of the genus Discosoma. It displays excitation and emission maxima of 554 and 581 nm, respectively. tdTomato is optimized for cell and tissue imaging, with improved fluorescence intensity and stability.

This mRNA features:
- A Cap 1 structure with high capping efficiency
- Codon optimized using GenScript X5 algorithm to enhance expression efficiency
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response
- GenScript’s patented UTR to enhance translation
- A 100A poly(A) tail to mimic natural mature mRNA

Properties

Form	Liquid
Concentration	1mg/mL
Full mRNA length	1696 nt
Full mRNA Molecular Weight	550032 Da
Storage buffer	1mM Sodium citrate, pH 6.5
Storage condition	Store at -20°C for short term (<3 months), store at -80°C for long term.

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Quality Control Specifications

Appearance	Clear and free of foreign particles
RNA Length	Expected size band detected
RNA Content	Target ± 5%
Integrity	≥ 75%
OD260/OD280	1.70 ~ 2.30
Capping Efficiency	≥ 90%
Endotoxin	< 10 EU/mg
pH	Target ± 0.5

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Applications

Transfect 0.14μg of tdTomato mRNA into one well of cells in a 96-well plate with Lipofectamine™ MessengerMAX™ (or equivalent). 24-48 hours after transfection, detect the expression of tdTomato protein by plate reader with excitation at 540nm and emission at 582nm.

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Expression of tdTomato mRNA in A549 cells. GenSmart, X4, and X5 represent GenScript mRNA codon optimized using different algorithms; ComP represents competitor P. »

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.