AAV Packaging Service

Highlight

7 BDs
to Deliver AAV

Industry-leading Turnaround Time

30+ AAV
Serotypes

Customizable for various special variants

100%
Accuracy

AAV ITR guaranteed QC available

≥1E+13 GC/mL
Guaranteed Delivery

Purity >95%
Full capsid ratio >70%

Workflow

Service Specifications

• Default QC

  SDS-Page

  

  Endotoxin

  

  qPCR

  
• Additional QC

  Mycoplasma Test

  

  TEM

  

  TCID₅₀

  

  Capsid titer

  

  Sanger Sequencing

  

  AUC

  

Case Study

• Case study 1: AAV purity test
  

  

  

  

  

  

  SDS-PAGE analysis of different AAV serotypes with silver staining

  (Purified virions were achieved by chromatography)

• Case study 2: AAV empty/full ratio detection
  

  Empty / Full Ratio

  Titer: 1x10^12-13 gc/ml (qPCR)

Resources

• Comparison of different viral vectors

  Characteristic	AAV	Retrovirus	Lentivirus
  Genome	4.8kb(ssDNA)	9.7kb (ssRNA)	9kb (ssRNA)
  Packaging Capacity	4.7kb	3kb	9kb
  Infection	Most dividing and non-dividing cells	Only infect mitotically active cells	Most dividing and non-dividing cells
  Transduction Efficiency	Moderate	High	Moderate
  Integration	Non-integrating	Integrating	Integrating
  Expression	Transient or stable	Stable	Stable or Transient
  Immunogenicity	Very Low	High	Low
  Biosafety	BSL-1	BSL-2	BSL-2
  Gene Therapy Strategy	In vivo & In vitro	Ex vivo	In vitro & Ex vivo

• AAV Serotype Recommendation

  Tissue type	Recommended AAV Serotype
  Central Nervous System (CNS)	AAV1, AAV5, AAV8, AAV9, AAV-DJ
  Heart	AAV1, AAV8, AAV9
  Kidney	AAV2, AAV9
  Liver	AAV8, AAV9, AAV-DJ
  Lung	AAV5, AAV6, AAV9
  Pancreas	AAV8
  RPE (Retinal Pigment Epithelium)	AAV1, AAV2, AAV5, AAV8
  Skeletal Muscle	AAV1, AAV6, AAV8, AAV9

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.

Related Service