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| $8000.00 | |
| RC00002-GMP | |
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| Ask us a question | |
CRISPR (clustered regularly interspaced short palindromic repeat) is an
adaptive immune system that provides protection against mobile genetic
elements (viruses, transposable elements and conjugative plasmids).
CRISPR clusters contain spacers, sequences complementary to antecedent
mobile elements, and target invading nucleic acids. CRISPR clusters are
transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR
systems correct processing of pre-crRNA requires a trans-encoded small
RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. |
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Genome editing with CRISPR T-Cells, Stem Cells, etc. |
| Appearance |
Clear liquid |
| Purity |
≥ 95.0% by RP-HPLC, Monomer ≥ 95.0%, Aggregates ≤ 5.0% by SEC-HPLC, ≥ 85.0% by NR-CE, ≥ 90.0% by R-CE |
| Quality |
GMP-Grade, Suitable for Manufacturing, Antibiotic-free and animal-free production |
| Concentration |
9.5-11.5 mg/mL |
| Bioactivity |
cleavage activity>85% |
| Residual DNase |
Negative |
| Residual RNase |
Negative |
| Endotoxin Level |
≤ 10.0EU/mg |
| Residual HCP |
≤ 100.0 ng/mL |
| Residual HCD |
≤ 3.0 ng/mL |
| Residual Nickel Salt |
≤ 10.0 ppm |
| pH |
7.4±0.5 |
| Synonyms |
CRISPR-associated endonuclease Cas9/Csn1, cas9, SpCas9, SpyCas9 |
| Source |
This product has undergone codon optimization, nuclear localization signal (NLS) design, and optimization of E. coli expression and purification. |
| Species |
Streptococcus pyogenes |
| Tag |
His tag |
| Molecular Weight |
163 kDa |
| Concentration |
10 mg/ml |
| Active temperature |
This Cas9 is active at 37°C. |
| Formulation |
Supplied as a solution of 30mM Tris-HCl, 0.3M NaCl, 50% Glycerol, 0.1 mM EDTA, pH 7.4 |
| Storage & Stability |
This product remains stable for up to 18 months at -20±5°C. Avoid repeated freeze-thaw cycles. |
Figure 1. Cas9 cuts substrate DNA standard via in vitro cutting experiment. Results show the cleavage activity of GenScript Cas9 is equivalent to that of leading competitors. »
Figure 2. The purity of GenScript Cas9 detected by SEC-HPLC is greater than 95%. »
Figure 3. The purity of Cas9 Nuclease detected by RP-HPLC was higher than 95% »
Figure 4. In vitro cleavage assays showed that the in vitro cleavage activity of Cas9 Nuclease was consistent across three batches, which are all higher than 85% »
Figure 5. Cas9 is electrotransfected into 293T cell line in the form of RNP to knock out the target gene. Results show GenScript Cas9 has the same knockout efficiency as leading competitors. »
Figure 6. Cas9 Nuclease is used for gene knockout in 293T and Jurkat cell lines. As shown in the figure, the gene knockout efficiency of the three batches of Kactus Cas9 Nuclease is comparable. »
