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| $150.00 | |
| RC00001-0.1 | |
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| Ask us a question | |
CRISPR (clustered regularly interspaced short palindromic repeat) is an
adaptive immune system that provides protection against mobile genetic
elements (viruses, transposable elements and conjugative plasmids).
CRISPR clusters contain spacers, sequences complementary to antecedent
mobile elements, and target invading nucleic acids. CRISPR clusters are
transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR
systems correct processing of pre-crRNA requires a trans-encoded small
RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
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Genome editing with CRISPR T-Cells, Stem Cells, etc. |
| Appearance | Clear liquid |
| Purity |
≥ 95.0% by SEC-HPLC, ≥ 95.0% by Bis-Tris PAGE |
| Quality |
Antibiotic-free and animal-free production |
| Concentration |
9.5-12.5 mg/mL |
| Bioactivity |
cleavage activity>85% |
| Residual RNase |
Negative |
| Endotoxin Level |
≤ 10.0EU/mg |
| Synonyms |
CRISPR-associated endonuclease Cas9/Csn1, cas9, SpCas9, SpyCas9 |
| Source |
This product has undergone codon optimization, nuclear localization signal (NLS) design, and optimization of E. coli expression and purification. |
| Species |
Streptococcus pyogenes |
| Tag | His tag |
| Molecular Weight |
163
kDa |
| Concentration |
10 mg/ml |
| Active temperature |
This Cas9 is active at 37°C. |
| Formulation |
Supplied as a solution of 30mM Tris-HCl, 0.3M NaCl, 50% Glycerol, 0.1 mM EDTA, pH 7.4 |
| Storage & Stability |
This product remains stable for up to 18 months at -20±5°C. Avoid repeated freeze-thaw cycles. |
Figure 1. Cas9 cuts substrate DNA standard via in vitro cutting experiment. Results show the cleavage activity of GenScript Cas9 is equivalent to that of leading competitors. »
Figure 2. The purity of GenScript Cas9 detected by SEC-HPLC is greater than 95%. »
Figure 3. The purity of GenScript Cas9 detected by Tris-Bis-PAGE is greater than 95%. »
Figure 4. In vitro cleavage assays showed that the in vitro cleavage activity of Cas9 Nuclease was consistent across three batches. »
Figure 5. Cas9 is electrotransfected into 293T cell line in the form of RNP to knock out the target gene. Results show GenScript Cas9 has the same knockout efficiency as leading competitors. »
