4 μg of lyophilized plasmid containing your gene insert (1 μg for low-copy plasmid) *
Sequence chromatograms or NGS read depth plot covering your gene (electronic)
Construct map for the plasmid (electronic)
Quality assurance certificate
- REAGENT SERVICES Hot!
-
PRODUCTS
-
Most Popular Reagents
-
Instruments
-
Antibodies
-
ELISA Kits
-
Protein Electrophoresis and Blotting
-
Protein and Antibody Purification
-
Recombinant Proteins
-
Molecular Biology
-
Stable Cell Lines
-
Cell Isolation and Activation
-
IVD Raw Materials
-
Therapy Applications
-
Resources
-
![AmMag™ Quatro Automated Plasmid Purification]()
AmMag™ Quatro automated plasmid purification
-
![Anti-Camelid VHH]()
MonoRab™ Anti-VHH Antibodies
-
![ELISA Kits]()
ELISA Kits
-
![Precast Gels]()
SurePAGE™ Precast Gels
-
![Quatro ProAb Automated Protein and Antibody Purification System]()
AmMag™ Quatro ProAb Automated Protein and Antibody Purification System
-
![Target Proteins]()
Target Proteins
-
![AmMag™ Quatro Automated Plasmid Purification]()
AmMag™ Quatro automated plasmid purification
-
![Stable Cell Lines]()
Stable Cell Lines
-
![Cell Isolation and Activation]()
Cell Isolation and Activation
-
![Quick
Order]()
Quick Order
-
![Quick
Order]()
Quick Order
- APPLICATIONS
- RESOURCES
- ABOUT US
![Homo sapiens (human) IGF1R signaling cascade (provided by Reactome)]( "Homo sapiens (human) IGF1R signaling cascade (provided by Reactome)")
Overview
Site-directed mutagenesis facilitates life science research by creating specific DNA mutations (including point mutations, deletion mutations, and insertion mutations) to enable studies of gene regulatory elements, DNA-protein interactions, protein structure/function, enzyme active sites, and novel proteins.
GenScript's site-directed mutagenesis services provide a fast, cost-saving, way to get 100% accurate mutant DNA clones, without having to spend your time and money on mutagenesis kits to do PCR mutagenesis, cloning, and sequencing in your own lab. Powered by our industry-leading gene synthesis technology and high-performance GS polymerase, GenScript can make any gene template and any number of mutations, starting at just $99/mutant and 5-day turnaround time.
Advantages of GenScript's Site-Directed Mutagenesis Services
Unparalleled Accuracy
GenScript's high performance GS polymerase ensures accuracy of mutants, avoiding creation of unwanted mutations.
Unlimited Sites
Using our sophisticated gene synthesis and mutagenesis techniques, GenScript can introduce mutations at any site.
Mutations on Large DNA Constructs
Our technology is optimized to introduce point mutations, insertion mutations, and deletion mutations on constructs as large as 12 kb (including target gene and its vector).
One-stop solutions
We offer comprehensive upstream and downstream services, including template DNA sequencing, de novo gene synthesis, Express Cloning into free expression vectors, customized vector construction, and protein expression and purification.
Most Competitive Prices
starting at $99/mutant.
High-Throughput Site-Directed Mutagenesis
Efficient / Low-Cost / High-Quality / 100% Accurate.Large Number of Individual Sequence-verified Mutants in an Arrayed Format
Mutagenesis Services Price and Turnaround Time
Express Mutagenesis bundled with gene synthesis+ Template Length Price Turnaround Time < 3 kb $99 per mutation* 5 business days 3 – 8 kb $99 per mutation* + $100 5 business days > 8 kb Request a quote Request a quote Mutagenesis from a template previously synthesized by GenScript- Template Length Price Turnaround Time < 3 kb $99 per mutation* Starts at 8 business days 3 – 8 kb $99 per mutation* + $100 > 8 kb Request a quote Site-Directed Mutagenesis from Customer-Provided Templates (no gene synthesis) Template length Price Turnaround Time < 1 kb $169 per mutation* 10 business days > 1 kb Starts at $269 Starts at 13 business days +Prices and turnaround times apply to mutagenesis ordered at the same time as new gene synthesis orders.
-For mutagenesis from a template previously synthesized by GenScript, please Quote with your previous order number to request a quote for price and turnaround time. Turnaround time may increase for inserts >1 kb.
*One mutation is defined as any combination of base changes within a 30-base frame.
Express Mutagenesis bundled with rocket gene synthesis+ Gene Length Price Turnaround* <3 kb $149 3 business days 3-8 kb $249 3 business days >8 kb Quote Quote +Prices and turnaround times shown for rocket gene synthesis apply to mutagenesis ordered at the same time as new gene synthesis orders.
How to Submit DNA templates for Mutagenesis
The fastest, and usually the most cost-effective, way to get the mutant DNA constructs you need is to order Express Mutagenesis bundled with gene synthesis using our online quotation form.
- The complete sequence of the template including the target gene and its vector.
- Mutation specifications.
- Maps and antibiotic resistance of the template and destination vectors.
- Recommended to send 4 μg of template DNA as the starting material. If the starting material is bacterial culture, please send the culture at sufficient amount.
Delivery Specifications for Mutagenesis Services
All of our mutagenesis services offer the following delivery package:
Quotations and Ordering
- For quotation requests and questions, you may contact us by phone, email, fax, or via our secured online quotation system. For batch orders of gene synthesis bundled with mutagenesis, please download our Quick Quotation Request Form.
- Orders can be placed by phone, email, fax, or online with a formal PO (Purchase Order) or credit card.
- To provide templates for mutagenesis (Cat No. SC1023), please send your samples with full shipping information and PO number (if applicable) with a hard copy of the completed Mutagenesis Quotation Request Form to Site-directed Mutagenesis Services, GenScript, 860 Centennial Avenue., Piscataway, NJ 08854, U.S.
Our customer service representatives are available 24 hours Monday through Friday.
You may contact us anytime for assistance.Customer Reviews
SERVICE MOBIOLOGY BIOLOGY -
