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T7 RNA Polymerase ELISA Kit

T7 RNA Polymerase ELISA Kit is used for detection and quantitative determination of T7 RNA Polymerase in the samples.
$1000.00
L01110

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Product Introduction
Product Description The kit is used for detection and quantitative determination of T7 RNA Polymerase in the samples. T7 RNA Polymerase ELISA kit uses the double antibody sandwich method: The micro plate has been pre-coated with anti-T7 RNA polymerase antibody. The sample is added and incubated with horseradish peroxidase (HRP) labeled antibody to form antibody-antigen-enzyme-labeled antibody complex. After washing, then TMB substrate solution is added and catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The depth of the color is positively correlated with the T7 RNA polymerase content in the sample. The absorbance (OD value) is measured at 450 nm.
Linearity Range 0.25-32 ng/mL
Detection Range Lower limit of detection: ＜0.1 ng/mL
Lower limit of quantitation: 0.25 ng/mL
Precision Intra-assay CV≤10%, Inter-assay CV≤15%
Recovery 80%-120%
Specificity only specific recognition of T7 RNA polymerase, other IVT tool enzymes do not interfere with the measurements.
Storage Unopened kit should be stored at 2-8°C for stability in shelf life.
Kit Contents
Component Quantity/Size
Capture Plate 1 plate
Standard Stock 50 μL
Enzyme Conjugate (100×) 80 μL
Sample Dilution Buffer 30 mL
Enzyme Conjugate Dilution Buffer 8 mL
Wash Solution (10×) 30 mL
Substrate Solution 12 mL
Stop Solution 6 mL
Plate Sealer 4 pieces
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.