Cas9 ELISA Kit

Cas9 ELISA Kit provides a rapid and quantitative solution for detecting Cas9 protein in cell lysates, viral vectors, and gene-editing reagents.

Price	$548.00
Cat. No.	L01069
Size	96 Tests Buy in bulk
Quantity
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Product Introduction Bottom Notes

Product Introduction

Linearity Range

0.125 to 8 ng/mL

Sensitivity

LLOQ：0.125ng/ml

Precision

Intra-assay: CV ≤ 10%;
Inter-assay: CV ≤ 15%

Accuracy

80%-120%

Standard

3.2 µg/mL of Cas9 protein

Suitable Sample Type

cell lysates, viral vectors, and gene-editing reagents

Specificity

This kit is designed for the detection of a wide range of Cas9 proteins, including wild-type SpCas9, high-fidelity mutants (e.g., eSpCas9), and variants featuring nuclear localization signals (NLS) at the N-terminus, C-terminus, or both.

Assay Temperature

37°C

Assay Time

3 h

Storage

The unopened kit is stable for 12 months from the date of manufacture at 2°C to 8°C, and the opened kit is stable for 1 month from the date of opening at 2°C to 8°C.

Kit Contents

Component	Quantity/Size	Part No.
Cas9 Capture Plate	1 plate	B2-80
Cas9 Standard Stock	1 vial (50 μL)	B2-10
Biotin Anti-Cas9 Antibody	1 bottle (12 mL)	B2-20
Streptavidin-HRP	1 bottle (12 mL)	B2-30
Sample Dilution Buffer	1 bottle (60 mL)	B2-60
20× Wash Solution	1 bottle (60 mL)	B-A1-71
TMB Solution	1 bottle (12 mL)	B-A1-40
Stop Solution	1 bottle (6 mL)	B-A1-50
Plate Sealer	2 pieces	/

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Figure 1. Typical Cas9 standard curve fitted with a four-parameter logistic model. »

Figure 2: Cas9 variants curves »

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.