Protein G MagBeads MX

Designed Use
GenScript Protein G MagBeads MX are ideal for small-scale antibody purification.

Principle
The sample containing antibody is added to the Protein G MagBeads MX. The antibody will bind to beads during a short incubation. Then the bead-bound antibody may be eluted off the beads by using a magnetic separation rack. Magnetic separation eliminates the need for multiple tubes, minimizes the loss of sample and removes tedious steps of conventional centrifugation method.

Material Supplied
GenScript Protein G MagBeads MX are superparamagnetic beads of average 45± 5 μm in diameter, covalently coated with recombinant Protein G. The beads are supplied as 25% slurry in 1 x PBS, pH 7.4, containing 20% ethanol. The Protein G MagBeads MX have a binding capacity of more than 30 mg Human IgG per 1 ml settled beads (e.g. 4 ml 25% slurry).
Protein G is a genetically engineered protein (MW≈22 kDa) that combines the IgG binding sites of both Protein A and Protein G. 6×His-tag was attached to its N-terminal to facilitate the purification. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.

Storage and Stability
This product is stable until the expiration date stated on the label, when stored unopened at 2–8°C. Do not freeze the product. Keep the MagBeads in liquid suspension during storage and all handling steps. Drying will cause loss of binding capacity and result in reduced performance. Resuspend the beads well before use. Be careful to avoid bacterial/fungal contamination.