His Tag ELISA Detection Kit

GenScript His Tag ELISA Detection Kit, a 1.5 hour competition ELISA, is developed for rapid and high throughput detection of His-tagged proteins.

Price	$328.00
Cat. No.	L00436
Size	1 Kit Buy in bulk
Quantity
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Product Introduction Bottom Notes

Note

L00436 interferes with IgG in mouse/rat serum and is not suitable for detecting mouse/rat serum samples.

Product Introduction

Product Description

GenScript His Tag ELISA Detection Kit, a 1.5 hour competition ELISA, is developed for rapid and high throughput detection of His-tagged proteins. There are several potential usages:
●Quickly identify the presence of His-tagged proteins in samples.
●Optimize protein expression by monitoring the His-tagged proteins level.
●High throughput screening of stable cell lines expressing His-tagged proteins.

Detection Range

1 ng/ml~729 ng/ml

Sensitivity

1 ng/ml His-tagged proteins

Test Samples

N-terminal/C-terminal/internal His-tagged proteins4 x His/5 x His/6 x His-tagged proteins
Mammalian, yeast and bacteria cell lysates or cell supernatant

Conveniency

Provide all reagents required for test
Complete test within 1.5 hours

Reagents compatibility

Tolerable with various reagents at certain concentration

Storage

The unopened kit is stable for 12 months if stored at 2-8 °C and the opened kit may be stable for 1 month at 2-8 °C. Do not freeze the kit.

Kit Contents

Component	Quantity	Part. No
His Tag Plate	1 plate (8 wells × 12 strips)	436-80
Anti-His Monoclonal Antibody	6 ml	436-20
Antibody Tracer	12 ml	436-30
His Tag Standards (0,1,3,9,27,81,243,729 ng/ml)	1 ml per each standard	436-11,12,13,14,15,16,17,18
Assay Diluent	60 ml	436-60
20 × Wash Solution	40 ml	B-A1-70
TMB Solution	12 ml	B-A1-40
Stop Solution	6 ml	B-A1-50
His Tag Standard Stock (10 μg/ml)	500 μl	436-10
Plate Sealer	2	N/A

Back

Concentration gradient represents X, OD450 value represents Y, all X values will be transformed to Log(X) to create the standard curve which was calculated by 4PL (Figure 1). The operator can get log values of sample concentration by inputting the OD450 value of unknown samples. By formula X=10^X, sample concentration can be calculated. »

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

Citations

1. Kaiwei Wang, et al. FlaB-expressing bacterial uter membrane vesicles vaccine mediating anti-tumor immune response via promoting DCs activation and lymph nodes-homing. Nano Research. (2025-08)
2. Jia Zheng, et al. High temperature delays and low temperature accelerates evolution of a new protein phenotype. Nat Commun. (2024-03)
3. Shi Hua Tan , et al. Thymosin β4 increases cardiac cell proliferation, cell engraftment, and the reparative potency of human induced-pluripotent stem cell-derived cardiomyocytes in a porcine model of acute myocardial infarction. Theranostics. (2022-06)
4. Mengshang Xiong, et al. A Novel CD3/BCMA Bispecific T-cell Redirecting Antibody for the Treatment of Multiple Myeloma. J Immunother. (2022-02)
5. Mengshang Xiong, et al. A Novel CD3/BCMA Bispecific T-cell Redirecting Antibody for the Treatment of Multiple Myeloma. J Immunother. (2022-02)
6. Xiaoxun Li, et al. CST6 protein and peptides inhibit breast cancer bone metastasis by suppressing CTSB activity and osteoclastogenesis. Theranostics. (2021-10)
7. Ye L, et al. OK/basigin expression on red blood cells varies between blood donors and correlates with binding of recombinant Plasmodium falciparum reticulocyte-binding protein homolog 5. Transfusion. (2018)