Ni-charged MagBeads

The GenScript Ni-Charged MagBeads are developed for quick and efficient small-scale purification of polyhistidine-tagged proteins under native or denaturing conditions.

Price	$101.00
Cat. No.	L00295
Size	8 ml (2 ml Settled Beads) Buy in bulk
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Overview Properties

Description

The GenScript Ni-Charged MagBeads are developed for quick and efficient small-scale purification of polyhistidine-tagged proteins under native or denaturing conditions.

Perform cell lysis under native or denaturing conditions. Add the cell lysate containing your polyhistidine-tagged proteins to the Ni-Charged MagBeads and allow the proteins bind to the MagBeads. Then the isolated proteins can be eluted from the beads. Magnetic separation eliminates the need for multiple tubes, minimizes the loss of sample and removes several steps of the centrifugation process.

The GenScript Ni-Charged MagBeads are average 40 µm in size, super paramagnetic beads with strong metal-chelating agent covalently bound to their surfaces. They are pre-charged with nickel and ready to use for quick and small-scale purification of polyhistidine-tagged proteins. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol and 1 mM NiSO₄. The Ni-Charged MagBeads have a binding capacity of 40 mg 6×His-tagged protein per 1 mL settled beads (e.g. 4 mL 25% slurry).

Overview

Key Features

Key Features

Quick and convenient separation accomplished by magnetic force
High binding capacity with 40 mg of 6xHis-tagged protein/ml settled beads
Compatible with various reagents needed in the purification process (see table 1)
Can be regenerated for multiple uses
Extremely low nonspecific binding

Table 1 Reagents Compatible with Ni-charged Magnetic Beads

Denaturants	Detergents	Reducing agents	Salts	Others
6 M Gu•HCl	2% Triton X-100	20 mM β-ME	4 M MgCl₂	50% glycerol
8 M Urea	2% Tween 20	1 mM DTT	5 mM CaCl₂	20% ethanol
	1% CHAPS		2 M NaCl	1 mM EDTA

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Properties

Storage & Stability

This product is stable until the expiration date stated on the COA, when stored unopened at 2–8°C. Do NOT freeze the product. Keep the MagBeads in liquid suspension during storage and all handling steps. Drying will cause loss of binding capacity and result in reduced performance. Resuspend the beads well before use. Be careful to avoid bacterial/fungal contamination.

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Citations

1. Brenna Norton-Baker, et al. Enabling high-throughput enzyme discovery and engineering with a low-cost, robot-assisted pipeline. Sci Rep. (2024-06)
2. Fei Liu, et al. Host Poly(A) Polymerases PAPD5 and PAPD7 Provide Two Layers of Protection That Ensure the Integrity and Stability of Hepatitis B Virus RNA. J Virol. (2021-08)
3. Shuanghong Yan , et al. Rapid and multiplex preparation of engineered Mycobacterium smegmatis porin A (MspA) nanopores for single molecule sensing and sequencing. Chem Sci. (2021-06)
4. Chen Q, et al. Exploring the Lower Limit of Individual DNA-Encoded Library Molecules in Selection. SLAS discovery : advancing life sciences R & D. (2020-06)
5. Jie Li, et al. A DNA-encoded library for the identification of natural product binders that modulate poly (ADP-ribose) polymerase 1, a validated anti-cancer target. Biochem Biophys Res Commun. (2020)