AmMag™ Quatro Maxi

Application Data

• Comparing Plasmid Extraction with AmMag™ Quatro Solution and Manual Kit

  Plasmid: pCDNA 3.1 (+) C-EGFP; Strain: DH5α; Culture Medium: TB; Culture Growth: 16 hours;
  Bacteria Pellet Weight: ~1.4 g (Quatro) ~0.4 g (Manual kit - maximum pallet used while not clogging the column)

  Sample	Extraction Method	Elution Volume	Concentration(ng/μL)	OD260/280	OD260/230	Endotoxin	Supercoiled Ratio
  1	Quatro	500 μl	947.01	1.86	2.33	<100 EU/mg	>90%
  2	Manual	500 μl	380.68	1.88	2.35	<100 EU/mg	>90%

  Test results show the AmMag™ Quatro solution yields equivalent amount of purified plasmid to a manual kit with high quality and low endotoxin levels.

• Transfection with AmMag™ Qautro Purified Plasmid
  

  pTT5-EGFP plasmid was purified using the AmMag™ Quatro solution, followed by transfection into the HEK 293T cell line. Transfection efficiency was assessed using flow cytometry to detect the expression levels of EGFP.

  The test results indicate that the number of cells expressing EGFP following transfection with the plasmid purified using the AmMag™ Quatro system was comparable to those obtained using the manual plasmid purification kit.

• Plasmid Extraction with AmMag™ Quatro Solution

  Plasmid: pCDNA 3.1; Strain: DH5α; Culture Medium: 100 ml TB; Culture Growth: 16 hours; Bacteria Pellet Weight: ~1.4 g; Elution Volume: 800 μl;

  Sample	Extraction Method	Concentration(ng/μL)	OD260/280	OD260/230	Endotoxin	Supercoiled Ratio
  1	Quatro	932.95	1.89	2.27	<100 EU/mg	>90%
  2	Quatro	949.65	1.88	2.36	<100 EU/mg	>90%
  3	Quatro	887.45	1.89	2.35	<100 EU/mg	>90%

  Test results show the AmMag™ Quatro solution yields high quality transfection-ready plasmids with low endotoxin levels.

Video

Service Plans

With a dedicated team of highly skilled and experienced engineers, GenScript delivers comprehensive warranty and maintenance services to ensure you get the best possible experience with our instruments.

Standard Warranty

One year warranty coverage of instrument repair, parts replacement, and technical support services, as frequent as needed.

Extended Warranty

Additional year-by-year extension coverage of instrument repair, parts replacement, and technical support services, as frequent as needed.

Planned Preventive Maintenance

Planned maintenance, preventive replacement of wearing parts, software update, and customizable maintenance plans, once per year.

Resources

GenScript’s AmMag™ Quatro Brochure

Automated large-scale, high-throughput plasmid purification solution

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GenScript’s AmMag™ Quatro Flyer

AmMag™ Quatro Maxi (1400) Purification Process

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Quatro Maxi Purification Process

AmMag™ Quatro Maxi (1400) Purification Process

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Quatro Maxi Virtual Demo Process

AmMag™ Quatro Maxi (1400) Virtual Demo Process

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AmMag™ Quatro 1400 Maxi-Scale Plasmid Purification

The AmMag™ Quatro 1400 system is designed for Maxi-Scale automated plasmid DNA purification.

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AmMag™ Quatro 1400 – Protocol Optimization Guide

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GenScript’s AmMag™ Quatro Webinar

Learn about automating Plasmid Maxi-prep with AmMag™ Quatro

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Unlock a New Era of Automated Mini- and Maxi-Scale Plasmid Purification with AmMag™ Quatro Solutions

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