THE™ His Tag Antibody [Biotin], mAb, Mouse

GenScript THE™ His Tag Antibody [Biotin], mAb, Mouse specificly recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

Price	$425.00
Cat. No.	A00613
Size	100 μg Buy in bulk
Quantity
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Overview Applications Properties Background Bottom Notes

Overview

Specificity	GenScript THE™ His Tag Antibody [Biotin], mAb, Mouse specificly recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Host Species	Mouse
Immunogen	A synthetic peptide (HHHHHH) coupled to KLH
Conjugate	Biotin

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Applications

Working concentrations for specific applications should be determined by the investigator.Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.02-0.2 µg/ml
Western Blot	0.5-1.0 µg/ml

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Properties

Form	Lyophilized
Storage Buffer	Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions	The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles.
Purification	Protein A affinity column
Isotype	Mouse IgG1,κ
Clonality	Monoclonal
Clone ID	6G2A9

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THE™ His Tag Antibody [Biotin], MAb, Mouse

Western blot analysis of His tagged fusion protein (Genscript, M0100) using THE^TM His Tag Antibody [Biotin], mAb, mouse (GenScript, A00613, 1 µg/ml)
The signal was developed with Streptavidin-HRP (GenScript, M00091, 1:10,000) and and LumiSensor^TM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 KD
Observed Size: 52 KD »

Background

Target Background	GenScript THE™ His Tag Antibody [Biotin], mAb, mouse is the biotin conjugated THE™ His Tag Antibody, mAb, mouse (A00186) purified from mice ascites by protein A affinity column.The antibody is suitable for detecting the expression level of His in fusion proteins.
Synonyms	THE™ His Tag Antibody [Biotin], mAb, Mouse;

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Citations

1. Yongbing Pan, et al. Screening of potent neutralizing antibodies against SARS-CoV-2 using convalescent patients-derived phage-display libraries. Cell Discov. (2021-07)
2. Temming AR, et al. Cross-reactivity of mouse IgG subclasses to human Fc gamma receptors: Antibody deglycosylation only eliminates IgG2b binding. Molecular immunology. (2020-11)
3. HussainKhiyam, et al. Impact of Human FcγR Gene Polymorphisms on IgG-Triggered Cytokine Release: Critical Importance of Cell Assay Format. Front Immunol. (2019-01)
4. QiXinyue, et al. Optimization of 4-1BB antibody for cancer immunotherapy by balancing agonistic strength with FcγR affinity. Nat Commun. (2019)
5. Sow HS, et al. FcγR interaction is not required for effective anti-PD-L1 immunotherapy but can add additional benefit depending on the tumor model. Int J Cancer. (2019)
6. V Hernandez-Gordillo, et al. Mimicking the extracellular matrix with functionalized, metal-assembled collagen peptide scaffolds. Biomaterials. (2014-08)
7. Fiona Wegman, et al. Gene delivery of bone morphogenetic protein-2 plasmid DNA promotes bone formation in a large animal model. J Tissue Eng Regen Med. (2012-08)