His-tag Antibody, pAb, Rabbit

GenScript His-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing His epitope tags.For N-terminal His-tagged protein detection, THE™ Anti-His mAb (Cat.No.A00186) is recommended.

Price	$102.00
Cat. No.	A00174-40
Size	40 μg Buy in bulk
Quantity
TAT


Ask us a question

Overview Applications Properties Background Bottom Notes

Overview

Specificity	GenScript His-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing His epitope tags.For N-terminal His-tagged protein detection, THE™ Anti-His mAb (Cat.No.A00186) is recommended.
Host Species	Rabbit
Immunogen	His epitope tag peptide HHHHHH conjugated to KLH
Conjugate	Unconjugated

Back

Applications

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.05-0.2 µg/ml
Western Blot	0.1-1.0 µg/ml
Western Blot	1:100-1:1,000

Back

Properties

Form	Lyophilized
Storage Buffer	Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions	The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles.
Purification	Affinity chromatography
Isotype	Rabbit IgG
Clonality	Polyclonal
Clone ID	Not applicable

Back

Background

Target Background	His-tag Antibody, pAb, Rabbit recognizes His tagged proteins in transfected or transformed cells.Short-sequence epitope tags are common in the study of protein expression in several different expression systems.In particular, the His tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography.
Synonyms	His-tag Antibody, pAb, Rabbit;

Back

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Citations

1. Kristen A Rodrigues, et al. Phosphate-mediated coanchoring of RBD immunogens and molecular adjuvants to alum potentiates humoral immunity against SARS-CoV-2. Sci Adv. (2021-12)
2. Ester Lopez, et al. Simultaneous evaluation of antibodies that inhibit SARS-CoV-2 variants via multiplex assay. JCI Insight. (2021-08)
3. Deli Huang, et al. A Rapid Assay for SARS-CoV-2 Neutralizing Antibodies That Is Insensitive to Antiretroviral Drugs. J Immunol. (2021-06)
4. Phu-Tri Tran, et al. Receptor-like kinase BAM1 facilitates early movement of the Tobacco mosaic virus. Commun Biol. (2021-04)
5. Zhao R, et al. A Membrane-Associated DHH-DHHA1 Nuclease Degrades Type III CRISPR Second Messenger. Cell Reports. (2020-09)
6. MacDonald C, et al. A Cycle of Ubiquitination Regulates Adaptor Function of the Nedd4-Family Ubiquitin Ligase Rsp5. Current biology : CB. (2020-02)
7. Matthew N. Idso, et al. Antibody-recruiting protein-catalyzed capture agents to combat antibiotic-resistant bacteria. Chem. Sci. (2020)
8. Steichen JM, et al. A generalized HIV vaccine design strategy for priming of broadly neutralizing antibody responses. Science. (2019-12)
9. WuMeng-Ju, et al. Epithelial-Mesenchymal Transition Directs Stem Cell Polarity via Regulation of Mitofusin. Cell Metab. (2019-04)
10. Kil LP1, et al. Restrained expansion of the recall germinal center response as biomarker of protection for influenza vaccination in mice. PLoS One. (2019)
11. BoopathyArchana V, et al. Enhancing humoral immunity via sustained-release implantable microneedle patch vaccination. Proc. Natl. Acad. Sci. U.S.A. (2019)
12. JeevaSubbiah, et al. Hantavirus RdRp Requires a Host Cell Factor for Cap Snatching. J. Virol. (2019)
13. Klingberg Franco, et al. The fibronectin ED-A domain enhances recruitment of latent TGF-β-binding protein-1 to the fibroblast matrix. J. Cell. Sci. (2018)
14. Chen XQ, et al. T-complex protein 1-ring complex enhances retrograde axonal transport by modulating tau phosphorylation. Traffic. (2018)
15. Tumurkhuu Gantsetseg, et al. Chlamydia pneumoniae Hijacks a Host Autoregulatory IL-1β Loop to Drive Foam Cell Formation and Accelerate Atherosclerosis. Cell Metab. (2018)
16. KulpDaniel W, et al. Structure-based design of native-like HIV-1 envelope trimers to silence non-neutralizing epitopes and eliminate CD4 binding. Nat Commun. (2017)
17. Pagliano C, et al. Use of Nicotiana tabacum transplastomic plants engineered to express a His-tagged CP47 for the isolation of functional photosystem II core complexes. Plant Physiol Biochem. (2017)
18. Liu Qingbo, et al. Quaternary contact in the initial interaction of CD4 with the HIV-1 envelope trimer. Nat. Struct. Mol. Biol. (2017)
19. Yang W, et al. Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization. Cell. (2016-11)
20. Philibert KD, et al. Identification and characterization of Aβ peptide interactors in Alzheimer's disease by structural approaches. Front Aging Neurosci. (2015)
21. Slomovic Shimyn, et al. DNA sense-and-respond protein modules for mammalian cells. Nat. Methods. (2015)
22. Jensen LD, et al. VEGF-B-Neuropilin-1 signaling is spatiotemporally indispensable for vascular and neuronal development in zebrafish. Proc Natl Acad Sci U S A. (2015)
23. Zhao H, et al. Foxp1/2/4 regulate endochondral ossification as a suppresser complex. Dev Biol. (2014-12)
24. Dan S, et al. A cell-free system toward deciphering the post-translational modification barcodes of Oct4 in different cellular contexts. Biochem Biophys Res Commun. (2014-12)
25. Sundlisaeter E, et al. The alarmin IL-33 is a notch target in quiescent endothelial cells. Am J Pathol. (2012-09)
26. Medici D, et al. Conversion of vascular endothelial cells into multipotent stem-like cells. Nat Med. (2010-12)
27. Lee JW, et al. The association of AMPK with ULK1 regulates autophagy. PLoS One. (2010-11)

Learn More

Back